Effects of probiotic lactobacilli on inflammatory responses to Escherichia coli in pig intestinal cells José D. Uezen, Jieyuan Jiang, María H. Miranda, María C. Aristimuño Ficoseco, Chengbo Yang, María E. F. Nader-Macias, Graciela M. Vignolo, Charles Martin Nyachoti

https://www.academia.edu/journals/academia-biology/articles?source=journal-top-nav Effects of probiotic lactobacilli on inflammatory responses to Escherichia coli in pig intestinal cells José D. Uezen [1], Jieyuan Jiang [2], María H. Miranda [1], María C. Aristimuño Ficoseco [1], Chengbo Yang [2], María E. F. Nader-Macias* [1], Graciela M. Vignolo [1], Charles Martin Nyachoti [2] https://www.academia.edu/2837-4010/3/4/10.20935/AcadBiol7982 In the swine industry, the increased awareness of the in-feed use of antibiotics due to the emergence of resistant bacteria led to an intense search for alternative strategies. Thus, to support swine optimal growth performance and gut health, dietary supplementation with probiotics was promisingly used. Here, porcine intestinal cells (IPEC-J2) were used to investigate the potential of probiotics Limosilactobacillus (Lim.) reuteri CRL2222, Lactobacillus (L.) amylovorus CRL2225, and Lactobacillus (L.) johnsonii CRL2229 to modulate innate immune functions and the intestinal barrier against injuries and pro-inflammatory reactions induced by ETEC F4 infection. By using qPCR, IPEC-J2 cell pre-treatment with each probiotic strain showed a significantly attenuated expression level of pro-inflammatory cytokines TNF-α, IL-8, and IL-6 during ETEC F4-induced infection. The gene expression of TLR4-mediated upstream related genes of the NF-κB signaling pathway (MyD88, IRAK-1, TRAF-6, and TAK-1) was significantly inhibited by the probiotic strains, resulting in the attenuation of inflammatory response in IPEC-J2 infected cells. Moreover, it was also revealed that Lim. reuteri CRL2222 and L. johnsonii CRL2229 probiotics increased the expression of zonula occludens 1 and occludin in ETEC F4-infected IPEC-J2 cells, alleviating the injury of epithelial barrier function. Therefore, these probiotics might be able to reduce pro-inflammatory cytokines blocking the NF-κB pathway through TLR4/MyD88 signaling and to prevent IPEC-J2 cells damage by enhancing the expression of tight-junction proteins. These results provide useful information on potential probiotics for the prevention/improvement of intestinal diseases in piglets.