Real-Time RT-PCR Panel for Detection 2019-Novel Coronavirus | NLM

Disaster Information Management Research Center | NLM

Real-Time RT-PCR Panel for Detection 2019-Novel Coronavirus

01/27/2020 12:00 AM EST

Source: Centers for Disease Control and Prevention, Office of Infectious Diseases (CDC OID). Published: 1/27/2020. This resource describes the use of real-time RT PCR (rT-PCR) assays for the in vitro qualitative detection of 2019-Novel Coronavirus (2019-nCoV) in respiratory specimens and sera. The 2019-nCoV primer and probe sets are designed for the universal detection of SARS-like coronaviruses and for specific detection of 2019-nCoV. Sections cover Specimens; Reagents, Supplies, and Equipment Requirements; Nucleic Acid Extraction; Quality Control; rRT-PCR Assays; Interpreting Test Results; Assay Limitations; and Contact Information. (Text)