Evaluation of Non–H. pylori Spiral Organisms in Human Gastric Biopsies by Using PCR and Microscopic Methods in Iran (First Report) European Scientific Journal ESJ

https://www.academia.edu/179383435/Evaluation_of_Non_H_pylori_Spiral_Organisms_in_Human_Gastric_Biopsies_by_Using_PCR_and_Microscopic_Methods_in_Iran_First_Report_ Introduction and Objectives: The Discovery of Helicobacter pylori in 1982 increased interest in the range of other spiral bacteria that had been seen in Stomach (Marshall & Warren 1984).The power of technologies such as the polymerase chain reaction (PCR) with genus specific primers revealed that many of these bacteria belong to the genus Helicobacter. These nonpylori helicobacters are increasingly being found in human clinical specimens. Non-pylori Helicobacters are Gram-negative, motile, long, tightly coiled, Spiral bacteria ,with three to eight coils, that cause of some like organisms(one of them kept a dog for 5 years as a pet).16S rRNA gene amplification was performed on 270 DNA samples and results were positive for H.heilmannii in two cases (275-bp), but negative for H.bizzozeronnii,H.felis and H. Salmonis.