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Chronic airway inflammation provides a unique environment for B cell activation and antibody production. - PubMed - NCBI

Chronic airway inflammation provides a unique environment for B cell activation and antibody production. - PubMed - NCBI



 2017 Apr;47(4):457-466. doi: 10.1111/cea.12878. Epub 2017 Jan 26.

Chronic airway inflammation provides a unique environment for B cell activation and antibody production.

Abstract

BACKGROUND:

B cells play many roles in health and disease. However, little is known about the mechanisms that drive B cell responses in the airways, especially in humans. Chronic rhinosinusitis (CRS) is an inflammatory disease of the upper airways that affects 10% of Europeans and Americans. A subset of CRS patients develop nasal polyps (NPs), which are characterized by type 2 inflammation, eosinophils and group 2 innate lymphoid cells (ILC2s). We have reported that NP contain elevated levels of B cells and antibodies, making NP an ideal system for studying B cells in the airways.

OBJECTIVE:

We sought to determine the mechanisms that drive B cell activation and antibody production during chronic airway inflammation.

METHODS:

We analysed B cells from NP or tonsil, or after ILC2 coculture, by flow cytometry. Antibody production from tissue was measured using Luminex assays and the frequency of antibody-secreting cells by ELISpot. Formation of B cell clusters was assessed using immunohistochemistry. Expression of genes associated with B cell activation and class switch recombination was measured by qRT-PCR.

RESULTS:

NP contained significantly elevated frequencies of plasmablasts, especially those that expressed the extrafollicular marker Epstein-Barr virus-induced protein 2 (EBI2), but significantly fewer germinal centre (GC) B cells compared with tonsil. Antibody production and the frequency of antibody-secreting cells were significantly elevated in NP, and there was evidence for local class switch recombination in NP. Finally, ILC2s directly induced EBI2 expression on B cells in vitro.

CONCLUSIONS AND CLINICAL RELEVANCE:

Our data suggest there is a unique B cell activation environment within NP that is distinct from classic GC-mediated mechanisms. We show for the first time that ILC2s directly induce EBI2 expression on B cells, indicating that ILC2s may play an important role in B cell responses. B cell-targeted therapies may provide new treatment options for CRSwNP.

KEYWORDS:

ENT ; B cells; IgE; lymphocytes

PMID:
 
28000955
 
PMCID:
 
PMC5378644
 
DOI:
 
10.1111/cea.12878

[Indexed for MEDLINE] 
Free PMC Article

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