Spotlight
Characterizing the fecal microbiota of infants with botulism. Infant botulism is the most prevalent form of botulism in the USA, representing 68.5 % of cases reported from 2001-2012. Infant botulism results when botulinum toxin-producing clostridia (BTPC) colonize the infant gut with concomitant in vivo production of the highly potent botulinum neurotoxin (BoNT). The gut microbiota of infants with botulism is largely uncharacterized; therefore, it remains unclear whether the microbiota profile of these patients are distinct in composition, abundance, or diversity. To address this uncertainty, we employed 16S rRNA gene profiling to characterize the fecal microbiota in 14 stool samples among laboratory-confirmed and non-confirmed infant botulism cases.... Significant differences in Proteobacteria, Firmicutes, and Enterobacteriaceae abundances were identified in the fecal microbiota of infants with botulism when compared to samples from non-confirmed cases. Fecal microbiota diversity was not significantly altered in infants with botulism, and a limited presence of BTPC was shown. It could not be determined whether the fecal microbiota profiles shown here were comparable prior to patient illness, or whether they were the direct result of infant botulism.
Advanced Molecular Detection Clips
Advanced Molecular Detection Clips Content
NEWS
- Data Storage on DNA Can Keep It Safe for Centuries
Markoff J. New York Times, Dec 3, 2015. - NIH Team Using MALDI Biotyper to Identify Antibiotic-Resistant Bacteria
Bonislawski A. GenomeWeb, Dec 7, 2015. - Swedish Study Evaluates BD Enteric Parasite Panel
Johnson M. GenomeWeb, Nov 25, 2015. - Disease-resistant pigs latest win for gene editing technology
Hirschler B. Reuters, Dec 8, 2015. - USAMRIID-Led Study Looks at Ebola Transmission, Adaptation in Liberia
Anderson A. GenomeWeb, Dec 9, 2015. - Liberia’s Ebola outbreak largely traced to one source
Saey TH. Science News, Dec 9, 2015.
POLICY
- Biosafety and biosecurity: a relative risk-based framework for safer, more secure, and sustainable laboratory capacity building
Dickman P, et al. Front Public Health, October 21, 2015.
CONCEPTS / COMMENTS
- Gut microbiota and aging
O'Toole PW, Jeffery IB. Science, Dec 4, 2015. - Ebola vaccine R&D: Filling the knowledge gaps
Medaglini D, et al. Sci Transl Med, Dec 9, 2015. - The Hologenome Concept: Helpful or Hollow?
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METHODS / TOOLS
- Revolutionising Public Health Reference Microbiology using Whole Genome Sequencing: Salmonella as an exemplar
Ashton P, et al. bioRxiv, Nov 29, 2015. - Wham: Identifying Structural Variants of Biological Consequence.
Kronenberg Zev N et al. PLoS computational biology 2015 Dec (12) e1004572 - Choice of reference-guided sequence assembler and SNP caller for analysis of Listeria monocytogenes short-read sequence data greatly influences rates of error.
Pightling Arthur W et al. BMC research notes 2015 748 - SBOL Visual: A Graphical Language for Genetic Designs.
Quinn Jacqueline Y et al. PLoS biology 2015 Dec (12) e1002310 - LAF: Logic Alignment Free and its application to bacterial genomes classification
Weitschek E, et al. BioData Mining 2015; 8: 39.
EVOLUTION / ECOLOGY / POPULATIONS
- Finished Annotated Genome Sequence of Burkholderia pseudomallei Strain Bp1651, a Multidrug-Resistant Clinical Isolate.
Bugrysheva Julia V et al. Genome announcements (6) (CDC Author) - Identification of new genotype of Echovirus 19 from children with Acute Flaccid Paralysis in Pakistan.
Angez Mehar et al. Scientific reports 2015 17456 - Comparative genomics of Pseudomonas fluorescens subclade III strains from human lungs.
Scales Brittan S et al. BMC genomics 2015 (1) 1032 - A viability-linked metagenomic analysis of cleanroom environments: eukarya, prokaryotes, and viruses.
Weinmaier Thomas et al. Microbiome 2015 (1) 62 - Evolution and Spread of Ebola Virus in Liberia, 2014–2015
Ladner JT, et al. Cell Host Microbe, Dec 9, 2015.
PATHOGENICITY / ANTIMICROBIAL RESISTANCE
- Transmission of HIV drug resistance and the predicted effect on current first-line regimens in Europe.
Hofstra L Marije et al. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2015 Nov - Full Genome Analysis of Enterovirus D-68 Strains Circulating in Alberta, Canada.
Pabbaraju Kanti et al. Journal of medical virology 2015 Dec - Clonal relationship between human and avian ciprofloxacin-resistant Escherichia coli isolates in North-Eastern Algeria.
Agabou A et al. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 2015 Dec
DETECTION / DIAGNOSIS
- Evaluation of the BD MAX™ Enteric Parasite Panel for clinical diagnostics.
Mölling Paula et al. Journal of clinical microbiology 2015 Nov - Comparative Analytical Evaluation of the Respiratory TaqMan Array Card with Real-Time PCR and Commercial Multi-Pathogen Assays.
Harvey John J et al. Journal of virological methods 2015 Nov (CDC Author) - Applying a PCR-based ORF typing method for easy genotyping and molecular epidemiological analysis of Pseudomonas aeruginosa.
Suzuki M et al. Journal of applied microbiology 2015 Dec - Evaluation of the BYG Carba test, a new electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae.
Bogaerts Pierre et al. Journal of clinical microbiology 2015 Dec - Optimization of the score cutoff value for routine identification of Staphylococcus species by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.
Han Huan Wen et al. Diagnostic microbiology and infectious disease 2015 Dec (4) 349-54 - Rapid antimicrobial susceptibility testing of clinical isolates by digital time-lapse microscopy.
Fredborg M et al. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 2015 Dec (12) 2385-94 - A Molecular Host Response Assay to Discriminate Between Sepsis and Infection-Negative Systemic Inflammation in Critically Ill Patients: Discovery and Validation in Independent Cohorts
McHugh L, et al. PLoS Med, Dec 8, 2015. - Targeted amplification for enhanced detection of biothreat agents by next-generation sequencing.
Gardner Shea N et al. BMC research notes 2015 (1) 682 - Making the Leap from Research Laboratory to Clinic: Challenges and Opportunities for Next-Generation Sequencing in Infectious Disease Diagnostics.
Goldberg Brittany et al. mBio 2015 (6) - Evaluation of MALDI-TOF Mass Spectrometry for the Identification of Mycobacterium species, Nocardia species and Other Aerobic Actinomycetes.
Buckwalter S P et al. Journal of clinical microbiology 2015 Dec - Comparative evaluation of the prototype Cepheid GeneXpert® Ebola Assay diagnostic performance.
van Vuren Petrus Jansen et al. Journal of clinical microbiology 2015 Dec - Deep sequencing of hepatitis B virus basal core promoter and precore mutants in HBeAg-positive chronic hepatitis B patients.
Yan Linlin et al. Scientific reports 2015 17950 - Trypanosoma brucei gambiense Spliced Leader RNA Is a More Specific Marker for Cure of Human African Trypanosomiasis Than T. b. gambiense DNA.
Ilboudo Hamidou et al. The Journal of infectious diseases 2015 Dec (12) 1996-8 - CCp5A Protein from Toxoplasma gondii as a Serological Marker of Oocyst-driven Infections in Humans and Domestic Animals.
Santana Silas S et al. Frontiers in microbiology 1305 - Innovative molecular diagnosis of Trichinella species based on β-carbonic anhydrase genomic sequence.
Zolfaghari Emameh Reza et al. Microbial biotechnology 2015 Dec - Molecular Differentiation of the African Yellow Fever Vector Aedes bromeliae (Diptera: Culicidae) from Its Sympatric Non-vector Sister Species, Aedes lilii.
Bennett Kelly Louise et al. PLoS neglected tropical diseases 2015 Dec (12) e0004250
EPIDEMIOLOGY / OUTBREAKS / TRANSMISSION
- Genomic Epidemiology of an Endoscope-Associated Outbreak of Klebsiella pneumoniae Carbapenemase (KPC)-Producing K. pneumoniae.
Marsh Jane W et al. PloS one 2015 (12) e0144310 - Next-Generation Sequencing and Comparative Analysis of Sequential Outbreaks Caused by Multidrug-Resistant Acinetobacter baumannii at A Large Academic Burn Center.
Kanamori Hajime et al. Antimicrobial agents and chemotherapy 2015 Dec
ANTIMICROBIALS / VACCINES / INTERVENTIONS
- Establishing a Fecal Microbiota Transplant Service for the treatment of Clostridium difficile Infection.
Costello Samuel P et al. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2015 Nov
HOST-MICROBE INTERACTIONS
- Characterizing the fecal microbiota of infants with botulism.
Shirey T Brian et al. Microbiome 2015 (1) 54 (CDC Author) - Disentangling type 2 diabetes and metformin treatment signatures in the human gut microbiota.
Forslund Kristoffer et al. Nature 2015 Dec - A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence.
Menachery Vineet D et al. Nature medicine 2015 Nov - Preliminary evaluation of exome sequencing to identify genetic markers of susceptibility to tuberculosis disease.
Duncan Carla et al. BMC research notes 2015 750 - Assessing the Intestinal Microbiota in the SHINE Trial.
Gough Ethan K et al. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2015 Dec S738-44 - Association of Single-Nucleotide Polymorphisms in IL28B, but Not TNF-α, With Severity of Disease Caused by Andes Virus.
Angulo Jenniffer et al. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2015 Dec (12) e62-9
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