lunes, 8 de diciembre de 2014

Familial hypercholesterolemia in Brazil: Cascade screening program,... - PubMed - NCBI

Familial hypercholesterolemia in Brazil: Cascade screening program,... - PubMed - NCBI

 2014 Nov 14;238(1):101-107. doi: 10.1016/j.atherosclerosis.2014.11.009. [Epub ahead of print]

Familial hypercholesterolemia in Brazil: Cascade screening program, clinical and genetic aspects.


Background: There is little knowledge about familial hypercholesterolemia in Brazil. This study presents the first results of genetic cascade screening performed in the city of Sao Paulo. Material and methods: Two-hundred and forty-eight suspected index cases were initially included. DNA was extracted from peripheral blood and the complete coding sequence of low-density lipoprotein receptor, exon 7 of proprotein convertase subtilisin/kexin type 9 gene and part of exon 26 of apolipoprotein B genes were sequenced. Multiplex Ligation-dependent Probe Amplification was performed on cases where a causal mutation was not identified through sequencing. After the identification of a causal mutation screening in first-degree relatives was pursued. Results: From 248 index cases, a mutation was found in 125 individuals (50.4%). 394 relatives were included in the cascade screening program and a mutation was identified in 59.4%. Seventy different causal mutations in the low-density lipoprotein receptor gene (97.2%) and 2 in the apolipoprotein B gene (2.8%) were found. No mutations were encountered in the proprotein convertase subtilisin/kexin type 9 gene. Mutations in exons 14 and 4 were the most prevalent and, 10 cases of true homozygotes (8 index cases and 2 relatives) and 1 compound heterozygote were identified. The most frequent mutation found was of Lebanese origin, the p.(Cys681*) mutation in exon 14 (8.5%). Conclusion: Genetic familial hypercholesterolemia cascade screening is feasible in Brazil and leads to identification of a mutation in approximately half of the index cases with higher rates of success in their relatives.
Copyright © 2014. Published by Elsevier Ireland Ltd.


Apolipoprotein B; Cascade screening; Familial hypercholesterolemia; Index patient; Low density lipoprotein receptor; Mutations

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