Jpn J Clin Oncol. 2018 Apr 12. doi: 10.1093/jjco/hyy052. [Epub ahead of print]
Feasibility study of cancer genome alterations identified by next generation sequencing: ABC study.
Naito Y1, Takahashi H1, Shitara K1, Okamoto W1, Bando H1, Kuwata T1, Kuboki Y1, Matsumoto S1, Miki I1, Yamanaka T2, Watanabe A3, Kojima M1.
Abstract
BACKGROUND:
To confirm the feasibility and explore the clinical applicability of amplicon sequencing by next generation sequencing (NGS) of biopsy samples from patients with advanced solid tumors, we conducted a prospective study.
METHODS:
Patients with unresectable, advanced, or recurrent solid tumors were included. Key eligibility criteria were as follows: 20 years or older, any planned systemic therapy, adequate lesion for biopsy, and written informed consent. Samples were fixed in 10% buffered formalin and embedded in paraffin. Cancer-derived DNA was extracted, and amplicon sequencing was performed using Ion AmpliseqTM Cancer Hotspot Panel version 1.0 or version 2.0 by central vendor. We evaluated the success rate of sequencing, and the proportion of the patients with actionable mutations. We organized an expert panel to share the results of targeted sequence, make annotations and reports, and discuss concomitant ethical/legal/social issues.
RESULTS:
A total of 232 patients were included, and 208 were successfully analyzed (success rate of 89.7%). The biopsy procedures were safe, with only one case of Grade 3 vasovagal reaction. The proportion of actionable/druggable mutations was 38.9% (81/208), which was not significantly different between the cancer panel version 1.0 and version 2.0 (P = 0.476). Expert panel could discuss the findings and make sufficient reports.
CONCLUSIONS:
We confirmed the feasibility of NGS-based amplicon sequencing using biopsy samples, making the basis for nationwide genome screening for cancer patients using biopsy samples. Our results suggest that focused panel may be sufficient to detect major mutations.
- PMID:
- 29659903
- DOI:
- 10.1093/jjco/hyy052
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